Alpha-Acetolactate Decarboxylase is produced by submerged fermentation of Bacillus subtilis followed by purification and formulation. It is widely used in food processing.
MECHANISM
Diacetyl, the α-acetolactate oxidative decomposition product, is an intermediate along the synthesis of amino acids in yeast, especially of valine and isoleucine. This decomposition process is very slow in yeast. Alpha-Acetolactate Decarboxylase can speed up the transformation of α-acetolactate into acetoin efficiently, avoiding the formation of the diketone, an off flavor in beer, and thereby greatly reducing diacetyl level in beer and diacetyl reduction time. In this way beer maturation time is saved.
REACTION PARAMETERS
CONDITION | RANGE |
Effective Temperature | 4℃-70℃ |
Effective pH | 3.0-7.5 |
PRODUCT SPECIFICATION
Light yellow liquid. Color may vary from batch to batch. Color intensity is not an indication of enzyme activity.
PRODUCT STANDARD
The product complies with GB1886.174.
No. | ITEMS | INDEX | |
1 | Bulk density/(g/ml) | 1.10-1.25 | |
2 | pH/(25℃) | 4.0-6.5 | |
3 | Lead/(mg/kg) | ≤5.0 | |
4 | Arsenic/(mg/kg) | ≤3.0 | |
5 | Total viable count/(CFU/mL) | ≤50000 | |
6 | Coliform Bacteria/(CFU/mL) | ≤30 | |
7 | Escherichia coli | (CFU/mL) | <10 |
(MPN/mL) | ≤3.0 | ||
8 | Salmonella/(25mL) | Not Detected |
APPLICATION RECOMMENDATION
The recommended dosage is 5-500g/t DS. The dosage has to be optimized based on each application, the raw material specifications, product expectation and processing parameters. It is better to begin the test with the convenient volume.
SAFE HANDLING PRECAUTIONS
Enzyme preparations are proteins that may induce sensitization and cause allergic type of symptoms in susceptible individuals. Prolonged contact may cause minor irritation for skin, eyes or nasal mucosa. Any direct contact with human body should be avoided. If irritation or allergic response for skin or eyes develops, please consult a doctor.
WARNINGS
Keep sealed after use every time to avoid microbial infections and inactivation of enzymes until its finish.
PACKAGE AND STORAGE
Ø Package: 25kg/drum.
Ø Storage: Keep sealed in a dry and cool place and avoid direct sunlight. Slight sedimentation is acceptable since it will not impact performance of the product.
Ø Shelf life: 12 months in a dry and cool place