Phytase enzyme for animal feed

Part I: Physical Characteristics and Specifications 

PRODUCT DESCRIPTION

 phytase is a highly concentrated thermostable feed grade phytase preparation. It breaks down the undigestible phytic acid (phytate) found in grains and oil seeds and thus release digestible phosphorus, calcium, and other nutrients. The thermostability, wide working pH range and the resistance to proteolysis of the product ensure its excellent performance. The even granulation and appropriate granulate size of the product make it easy to blend with feed well. 

As an animal feed additive often used in poultry and swine feed, it enhances the nutritive value of plant material in feed by liberation of inorganic phosphate from phytic acid (myo-inositol hexakisphosphate) , thereby, reduce environmental phosphorus pollution.

SPECIFICATIONS 

 *Color can vary from batch to batch. Color intensity is not an indication of enzyme activity. 

**1 unit of phytase equals to the amount of enzyme, which liberates 1 μmol of inorganic phosphorus in 1 min, from 5.0mmol/L sodium phytase at 37℃ and pH5.0 

 ENZYME PROPERTIES

 pH profile

Heat stability

 RECOMMENDED DOSAGE    

 Dilute the product before use. 

BENEFITS 

1. Effectively hydrolyze phytic acid in the plant materials, increasing the phosphorus utilization rate of feed raw material.
2. Reduce the dosage of inorganic phosphorus, saving cost and feed formulation space.
3. Break phytanate frame, releasing nutrients like starch, protein, minerals and increasing the utilization rate.
4. Liberate phosphorus from phytate in plant materials, balancing the metabolic role of animals.
5. Improve the absorption of nitrogen and phosphorus and reduce environmental pollution.

SAFE HANDLING PRECAUTIONS 

Enzyme preparations are proteins that may induce sensitization and cause allergic type of symptoms in susceptible individuals. Prolonged contact may cause minor irritation for skin, eyes or nasal mucosa. Any direct contact with human body should be avoided. If irritation or allergic response for skin or eyes develops, please consult a doctor. 

WARNINGS 

Keep sealed after use every time to avoid microbial infections and inactivation of enzymes until its finish. 

PACKAGE AND STORAGE 

Ø  Package： 25kg/bag

Ø  Storage： Keep sealed in a dry and cool place and avoid direct sunlight.

Ø  Shelf life:  12 months in a dry and cool place (20℃).   

Part II: Stability Analysis of Phytase 

1. Storage stability (shelf life) 

Storage stability test of Phytase was conducted under the following conditions:

Storage time: 12 months

Tested batch: 2020034501

Storage conditions: The batch was stored in controlled humidity and temperature chamber at temperature 25±2℃ and relative humidity of 60±5%.

Parameters evaluated: Appearance, Colour, Activity, Loss on drying 

Control: Identical product. 

Results 

The results shown in the above table shows quite good storage stability under the specified storage condition. 

2. Pelleting stability

The stability of the phytase was also evaluated in pelleting process. The enzyme was mixed evenly with feed before pelleting. The table below summarizes the activity loss due to pelleting.  

On basis of the results obtained from pelleting , following conclusions could be drawn:
1. At pelleting temperatures exceeding 80℃ it is recommended that 15% more phytase should be added to ensure the targeted activity in the final feed pellets.
2. Even distribution of the phytase must be secured.

Part III: Application guidance for Phytase  

Application 1: Mixing recommended dosage directly with complete feed

Mixing recommended dosage of phytase into 1 ton of complete feed can substitute 12 kgs of calcium hydrophosphate containing 16% of P. At the same time, mixing limestone to supplement the content of Ca in calcium hydrophosphate (assumption: the content of Ca in calcium hydrophosphate is 23%，in limestone is 38%, the amount of limestone should be added is 12×23%÷38%=7.26kg/ton). The gap (12-7.26-0.2=4.72) can be bridged by other ingredients like corn, wheat bran or zeolite.

Application 2: Based on potential P content to be released by phytase

Input the potential P content liberated by phytase into the calculation model, set the maximum dosage of phytase, and set the specification for total content of P as 0.45~0.60%, then calculate the best formula by the model.

Application 3: According to all the potential nutrients made available by phytase

Count all of the potential nutritional data (including metabolic energy, crude protein, amino acid, Ca, total P, available P etc.) made available by phytase into calculation model, set the maximum dosage of phytase, then calculate the best recipe by the model without any adjustment to specification of feed.  

Application 4: Ascertain the mixing amount of phytase and substitution amount of calcium hydrophosphate according to the actual content of phytic acid   P in feed formula

Please kindly note that 150 u of phytase is needed to release one gram of phytic acid P .

Step 1: Calculate the actual content of phytic acid P in feed recipe.

Step 2: Calculate the amount of phytase to make up the gap in P content. 

Step 3: Calculate the content of the potentially available P released by phytase.

Step 4: Calculate the substitution amount of calcium hydrophosphate according to the content of available P released by phytase and the content of P in calcium hydrophosphate.

Step 5: Calculate the supplement amount of limestone due to reduction of calcium hydrophosphate according to the content of Ca in calcium hydrophosphate and limestone.

Step 6: The gap can be bridged by other ingredients like corn, wheat bran or zeolite.  

Part IV: Performance of Phytase  

1. Used for laying hens:

120-150g/MT of complete layer feed of phytase can normally substitute 12kgs of calcium hydrophosphate containing 16% of P, at the same time, 7.26kgs of limestone having 38% of Ca and 4.73kgs of corn are needed, then:

6. Cost of feedstuff is reduced by RMB6.00/ton;
7. Metabolic Energy (ME) is increased by 15.6kcal/kg;
8. Taking Lohammann layer as example, egg production rate can be increased by 3.25%, metabolizability rate can be increased by 3.17%, ratio of feedstuff and egg can be decreased by 2.74%, average egg weight can be increased by 2.16%.
9. Used for growing hens: 

Input the potential available P released by phytase into the recipe calculation model, and set the maximum dosage of phytase as 0.015%, decrease the requirement of total P in recipe to 0.40%, then calculate the best formula with the calculation model, the cost of feedstuff can be reduced by RMB8.50/ton.

Mixing 300u of phytase with 1kg of layer’s diet can improve layer’s tibia intensity and bone ash weight significantly, decrease the total content of added P in diets and has no negative effect on the content of Ca,P,Cu,Mn,Zn in bone. 

3. Used for breeding layer:

Input all the amount of potential nutrients (including ME, CP, amino acid, Ca, total P, available P etc.) made available by phytase into the calculation model, and set the maximum dosage of phytase as 0.015%, set the nutrient specification of feed the same as that of control group, then calculate the best formula with calculation model, the cost of feedstuff can be reduced by RMB10.50/ton. The result is as follows:

Mixing 120-150g of phytase per ton of feed for breeding layer’s diets can enhance incubation rate of chicks significanty, improve the egg production rate, feed intake and impregnation. 

4. Used for broiler and meat duck

If 1 ton of complete feed for broiler and meat duck is mixed with 150-200gs of phytase to substitute 12kgs of calcium hydrophosphate having 16% of P, at the same time, 7.26kgs of limestone with 38% of Ca and 4.72kgs of corn are supplemented, cost of feedstuff can be reduced by RMB6.00/ton. Or

Input the potentially available P released by phytase into the calculation model, and set the maximum dosage of phytase as 0.02%, decrease the requirement of total P in formula to 0.60%, then calculate the best formula by calculation model, the cost of feedstuff can be reduced by RMB6.50/ton.

5. Used for pigs

If 1 ton of complete feed for pigs is mixed with 150-200gs of phytase to substitute 10kgs of calcium hydrophosphate having 16% of P, at the same time, 6.05kgs of limestone with 38% of Ca and 3.93kgs of corn are supplemented, cost of feedstuff can be reduced by RMB5.00/ton and metabolic energy can be increased by 13.2kcal/kg.

  Part V: Recommended Dosage of Phytase 

Recommendation of Dosage on basis of Feedstuff 

Notes for the dosing:

1. Dilute phytase in portion of 1:10, then mix it with complete feed.
2. Dilute phytase in portion of 1:5, then mix it with concentrate.
3. Directly add into premix.
4.     Stepwise mixing into feed to ensure even distribution.

Sample Formula of Phytase